FAQs — Hypersil GOLD Amino Column
Q: What is the Hypersil GOLD Amino column and what is it used for?
Hypersil GOLD Amino is a high-performance aminopropyl HPLC column engineered for excellent chromatographic properties across three operating modes: weak anion exchange, reversed-phase, and normal phase. It is designed for sugar and carbohydrate analysis, organic acid and anion separations, normal-phase lipid and vitamin analysis, and any application requiring the versatility of a tri-mode aminopropyl stationary phase with outstanding peak shape and sensitivity.
Q: What are the three operating modes of Hypersil GOLD Amino?
Hypersil GOLD Amino operates in three modes: (1) weak anion exchange — where the protonated aminopropyl group retains anions and organic acids via electrostatic interaction; (2) reversed-phase — where the phase is excellent for carbohydrate and sugar analysis, retaining polar analytes that are undetained on C18; and (3) normal phase — where it provides alternative selectivity to silica columns with improved reproducibility. The mobile phase composition determines which mode is active.
Q: Why is Hypersil GOLD Amino recommended for carbohydrate analysis?
Carbohydrates and sugars are highly polar and are essentially unretained on standard reversed-phase C18 columns. The aminopropyl phase provides polar interaction mechanisms that retain sugars under high-organic-content mobile phases (e.g., 80:20 acetonitrile:water), delivering retention, resolution, and excellent peak shape for common sugars including fructose, glucose, sucrose, maltose, and lactose.
Q: What analytes were demonstrated in the sugars application chromatogram?
Five common sugars were separated: Fructose, Glucose, Sucrose, Maltose, and Lactose — on Hypersil GOLD Amino, 5 µm, 150 × 4.6 mm, using 80:20 MeCN:Water mobile phase at 1.2 mL/min, 35°C, with RI detection. All conditions are sourced directly from the manufacturer's application chromatogram legend.
Q: What method conditions were used in the sugars chromatogram?
Column — Hypersil GOLD Amino, 5 µm, 150 × 4.6 mm; Mobile Phase — MeCN : Water (80:20); Temperature — 35°C; Flow Rate — 1.2 mL/min; Injection Volume — 20 µL; Detection — RI; Analytes — Fructose, Glucose, Sucrose, Maltose, Lactose. All values are sourced directly from the manufacturer's application chromatogram legend.
Q: How does Hypersil GOLD Amino provide alternative selectivity to silica in normal-phase mode?
In normal-phase mode, the aminopropyl functional group provides polar interaction selectivity that differs from the purely silanol-based interaction of bare silica columns. This alternative selectivity allows resolution of compound classes that are poorly differentiated on silica, while also offering better reproducibility — bare silica activity is strongly affected by water content of mobile phases, whereas the bonded aminopropyl phase is more stable and consistent.
Q: What USP classification does Hypersil GOLD Amino carry?
Hypersil GOLD Amino is classified under USP L8, as stated in the manufacturer's product literature. This supports its use in pharmacopoeial methods and regulated laboratory workflows specifying a USP L8 aminopropyl stationary phase.
Q: What are the pore size, carbon load, and pH range of Hypersil GOLD Amino?
Hypersil GOLD Amino has a pore size of 175 Å, a carbon load of approximately 2% (low, as indicated in the manufacturer's phase property chart), and a pH operating range of 2 to 8. Always refer to the column care and use instructions supplied with your column for precise operating limits.
Q: What particle sizes are available for Hypersil GOLD Amino?
Hypersil GOLD Amino is available in three analytical particle sizes: 1.9 µm (UHPLC format), 3 µm (HPLC), and 5 µm (HPLC) — covering fast UHPLC methods through to standard analytical HPLC separations.
Q: What column lengths and internal diameters are available for Hypersil GOLD Amino?
The 1.9 µm UHPLC format is available in 50–200 mm lengths in 2.1 mm ID only. The 3 µm format covers 30–150 mm lengths across 1.0, 2.1, 3.0, and 4.6 mm ID. The 5 µm format covers 50–250 mm lengths across 2.1, 3.0, 4.0, and 4.6 mm ID. Not all combinations are available for every particle size — see the Variants & Ordering tab for the complete matrix.
Q: Is a guard column available for Hypersil GOLD Amino?
Yes. Drop-in Guard cartridges (4/pk, 10 mm) are available for 3 µm columns in 1.0, 2.1, 3.0, and 4.6 mm ID, and for 5 µm columns in 2.1, 3.0, 4.0, and 4.6 mm ID. Uniguard Drop-in Guard Cartridge Holders are available: 851-00 (1.0 mm ID), 852-00 (2.1 mm / 3.0 mm ID), and 850-00 (4.0 mm / 4.6 mm ID).
Q: Where can I buy Hypersil GOLD Amino columns in Maharashtra?
Hypersil GOLD Amino columns are available in Maharashtra through Subodh Trading Company, the authorized Thermo Fisher distributor for Maharashtra. Subodh Trading Company supplies genuine Thermo Fisher products with full warranty and Certificate of Analysis, serving laboratories across Pune, Nagpur, Nashik, Aurangabad, Chhatrapati Sambhaji Nagar, Solapur, Sangli, Kolhapur, Satara, Ahmednagar, Jalgaon, Nanded, Amravati, Akola, Latur, Ratnagiri, Thane, and all of Maharashtra. Contact: sales@subodhtrading.com.
Q: How do I obtain a Certificate of Analysis for my Hypersil GOLD Amino column?
A Certificate of Analysis is available for every Hypersil GOLD Amino column by part number and lot number from the Thermo Fisher CoA search portal at thermofisher.com. Subodh Trading Company can also assist customers in Maharashtra — email sales@subodhtrading.com with your purchase details or column lot number.
Hypersil GOLD Amino — 1.9, 3, and 5 µm particle sizes, USP L8, 175 Å pore size, pH 2–8.
Hypersil GOLD Amino, 5 µm, 150 × 4.6 mm. Mobile Phase: MeCN : Water (80:20). Temperature: 35°C. Flow Rate: 1.2 mL/min. Injection Volume: 20 µL. Detection: RI.
