FAQs — Hypersil GOLD AX Column
Q: What is the Hypersil GOLD AX column and what is it used for?
Hypersil GOLD AX is a weak anion exchange HPLC column based on a novel polymeric amine ligand bonded to highly pure base-deactivated silica — designed for weak anion exchange retention of multiple charged species and HILIC-mode retention and separation of highly polar molecules. It is particularly suited for Vitamin C (ascorbic acid) analysis, organic and inorganic anion profiling, multiply charged pharmaceutical ion analysis, and highly polar analytes that are not retained on standard reversed-phase columns.
Q: What makes Hypersil GOLD AX different from standard polymer-based ion exchange columns?
Hypersil GOLD AX is engineered for higher efficiency than polymer-based ion exchange columns. The polymeric amine ligand is bonded to highly pure base-deactivated silica — a particle platform that delivers significantly better efficiency and peak shape than traditional polymer bead-based anion exchange columns. This translates to sharper peaks, better resolution, and more reliable quantification for the same analyte classes.
Q: What is the novel polymeric amine ligand in Hypersil GOLD AX?
The Hypersil GOLD AX stationary phase uses a novel polymeric amine ligand — a multi-amine functional polymer structure bonded to the silica surface. This polymeric amine architecture provides weak anion exchange retention through multiple protonatable amine groups, while the polymeric nature of the ligand also supports HILIC-mode retention for highly polar molecules through interaction with the hydrophilic amine surface.
Q: Can Hypersil GOLD AX operate in HILIC mode?
Yes. Hypersil GOLD AX is described in manufacturer literature as suitable for HILIC retention and separation of highly polar molecules. The polar polymeric amine surface supports the formation of a water-enriched layer under high-organic mobile phase conditions — enabling retention of highly polar and zwitterionic analytes that are unretained on both reversed-phase and standard anion exchange phases.
Q: What was demonstrated in the Vitamin C application chromatogram?
The application chromatogram demonstrates Hypersil GOLD AX retaining and separating Vitamin C (ascorbic acid) — a highly polar, charged organic acid — with a sharp, well-resolved peak using 100 mM Ammonium Acetate, pH 4.8 mobile phase with UV detection at 249 nm on a 5 µm, 100 × 4.6 mm column. All conditions are sourced directly from the manufacturer's application chromatogram legend.
Q: What particle sizes are available for Hypersil GOLD AX?
Hypersil GOLD AX is available in three analytical particle sizes: 1.9 µm (UHPLC format), 3 µm (HPLC), and 5 µm (HPLC) — covering fast UHPLC methods through to standard analytical HPLC separations.
Q: What pore size does Hypersil GOLD AX use?
Hypersil GOLD AX has a pore size of approximately 100 Å, as indicated in the manufacturer's phase property chart. This pore size is appropriate for small molecule anion exchange and HILIC applications targeting low molecular weight charged species and polar molecules.
Q: What column lengths and internal diameters are available for Hypersil GOLD AX?
The 1.9 µm UHPLC format is available in 50–200 mm lengths in 2.1 mm ID only. The 3 µm format covers 30–150 mm lengths across 1.0, 2.1, 3.0, and 4.6 mm ID. The 5 µm format covers 50–250 mm lengths across 2.1, 3.0, and 4.6 mm ID. Note that most 5 µm HPLC column lengths (50, 100, 150 mm) are currently listed only in 4.6 mm ID. See the Variants & Ordering tab for the complete part number matrix.
Q: Is a guard column available for Hypersil GOLD AX?
Yes. Drop-in Guard cartridges (4/pk, 10 mm) are available for 3 µm columns in 1.0, 2.1, and 4.6 mm ID, and for 5 µm columns in 2.1, 3.0, and 4.6 mm ID. Uniguard Drop-in Guard Cartridge Holders are available: 851-00 (1.0 mm ID), 852-00 (2.1 mm / 3.0 mm ID), and 850-00 (4.6 mm ID).
Q: What are the part numbers for the 100 mm Hypersil GOLD AX columns?
The 100 mm Hypersil GOLD AX columns are available under: 26102-102130 (1.9 µm, 2.1 mm ID), 26103-102130 (3 µm, 2.1 mm ID), 26103-104630 (3 µm, 4.6 mm ID), and 26105-104630 (5 µm, 4.6 mm ID). All part numbers are sourced directly from manufacturer product literature.
Q: Where can I buy Hypersil GOLD AX columns in Maharashtra?
Hypersil GOLD AX columns are available in Maharashtra through Subodh Trading Company, the authorized Thermo Fisher distributor for Maharashtra. Subodh Trading Company supplies genuine Thermo Fisher products with full warranty and Certificate of Analysis, serving laboratories across Pune, Nagpur, Nashik, Aurangabad, Chhatrapati Sambhaji Nagar, Solapur, Sangli, Kolhapur, Satara, Ahmednagar, Jalgaon, Nanded, Amravati, Akola, Latur, Ratnagiri, Thane, and all of Maharashtra. Contact: sales@subodhtrading.com.
Q: How do I obtain a Certificate of Analysis for my Hypersil GOLD AX column?
A Certificate of Analysis is available for every Hypersil GOLD AX column by part number and lot number from the Thermo Fisher CoA search portal at thermofisher.com. Subodh Trading Company can also assist customers in Maharashtra — email sales@subodhtrading.com with your purchase details or column lot number.
Hypersil GOLD AX — Novel polymeric amine ligand on highly pure base-deactivated silica. 1.9, 3, and 5 µm particle sizes, ~100 Å pore size.
Hypersil GOLD AX, 5 µm, 100 × 4.6 mm. Mobile Phase: 100 mM Ammonium Acetate, pH 4.8. Detection: UV 249 nm.
