FAQs — Hypersil GOLD C4 Column
Q: What is the Hypersil GOLD C4 column and what is it used for?
Hypersil GOLD C4 is an endcapped, ultra-pure silica reversed-phase HPLC column engineered for very hydrophobic analytes — compound classes where lower hydrophobicity than C18 or C8 is required to achieve practical retention, faster separations, and excellent peak shape. It is particularly well suited for fatty acid analysis, lipid separations, highly lipophilic pharmaceutical compounds, and large hydrophobic molecules.
Q: Why choose C4 over C8 or C18 for very hydrophobic analytes?
Very hydrophobic analytes — such as fatty acids, lipids, and large hydrophobic biomolecules — are often over-retained on C18 and C8 phases, leading to impractically long run times, broad peaks, and high organic solvent requirements. The shorter C4 alkyl chain provides lower hydrophobicity, bringing these analytes into a more manageable retention window for faster, better-resolved separations under practical mobile phase conditions.
Q: How does Hypersil GOLD C4 compare to Hypersil GOLD C8 and C18?
All three columns are built on the same ultra-pure silica endcapping platform and deliver the same excellent peak shape, high efficiency, and outstanding sensitivity. The difference is hydrophobicity: C18 is highest, C8 is moderate, and C4 is lowest — making C4 the correct choice when analyte lipophilicity is so high that C8 still over-retains. Selectivity order is generally preserved across the three phases, enabling straightforward method transfer.
Q: What was demonstrated in the fatty acids application chromatogram?
The fatty acids application demonstrates the separation of Linolenic acid and Linoleic acid on Hypersil GOLD C4, 1.9 µm, 100 × 2.1 mm, using 110:2 ACN : 20 mM formate mobile phase at 0.5 mL/min, 30°C, with UV detection at 205 nm. All conditions are sourced directly from the manufacturer's application chromatogram legend.
Q: What particle sizes are available for Hypersil GOLD C4?
Hypersil GOLD C4 is available in three analytical particle sizes: 1.9 µm (UHPLC format), 3 µm (HPLC), and 5 µm (HPLC) — covering fast UHPLC sub-minute methods through to standard analytical HPLC separations.
Q: What USP classification does Hypersil GOLD C4 carry?
Hypersil GOLD C4 is classified under USP L26, as stated in the manufacturer's product literature. This supports its use in pharmacopoeial methods and regulated laboratory workflows specifying a USP L26 C4 stationary phase.
Q: What is the pore size of Hypersil GOLD C4 and why is it larger than the C18 and C8 phases?
Hypersil GOLD C4 has a pore size of approximately 300 Å — larger than the ~150 Å pore of the Hypersil GOLD C18 and C8. A larger pore size improves access to the stationary phase for larger hydrophobic molecules such as lipids, fatty acids, and hydrophobic peptides, which may have restricted access in smaller-pore phases.
Q: What pH range does Hypersil GOLD C4 operate within?
Hypersil GOLD C4 is designed to operate across a pH range of approximately 2 to 11, as indicated in the manufacturer's phase property chart. Always refer to the column care and use instructions supplied with your column for precise operating limits.
Q: What column lengths and internal diameters are available for Hypersil GOLD C4?
The 1.9 µm UHPLC format is available in 50–150 mm lengths across 1.0 and 2.1 mm ID. The 3 µm format covers 50–150 mm lengths across 1.0, 2.1, 3.0, and 4.6 mm ID. The 5 µm format covers 50–250 mm lengths across 2.1, 3.0, and 4.6 mm ID. Not all combinations are available for every particle size — see the Variants & Ordering tab for the complete matrix.
Q: Is a guard column available for Hypersil GOLD C4?
Yes. Drop-in Guard cartridges (4/pk, 10 mm) are available for 3 µm columns across 1.0, 2.1, 3.0, and 4.6 mm ID, and for 5 µm columns in 2.1 and 4.6 mm ID. Uniguard Drop-in Guard Cartridge Holders are available: 851-00 (1.0 mm ID), 852-00 (2.1 mm / 3.0 mm ID), and 850-00 (4.6 mm ID).
Q: What are the part numbers for the 100 mm Hypersil GOLD C4 columns in 5 µm?
The 100 mm Hypersil GOLD C4, 5 µm HPLC columns are available under: 25505-102130 (2.1 mm ID), 25505-103030 (3.0 mm ID), and 25505-104630 (4.6 mm ID). All part numbers are sourced directly from manufacturer product literature.
Q: Where can I buy Hypersil GOLD C4 columns in Maharashtra?
Hypersil GOLD C4 columns are available in Maharashtra through Subodh Trading Company, the authorized Thermo Fisher distributor for Maharashtra. Subodh Trading Company supplies genuine Thermo Fisher products with full warranty and Certificate of Analysis, serving laboratories across Pune, Nagpur, Nashik, Aurangabad, Chhatrapati Sambhaji Nagar, Solapur, Sangli, Kolhapur, Satara, Ahmednagar, Jalgaon, Nanded, Amravati, Akola, Latur, Ratnagiri, Thane, and all of Maharashtra. Contact: sales@subodhtrading.com.
Q: How do I obtain a Certificate of Analysis for my Hypersil GOLD C4 column?
A Certificate of Analysis is available for every Hypersil GOLD C4 column by part number and lot number from the Thermo Fisher CoA search portal at thermofisher.com. Subodh Trading Company can also assist customers in Maharashtra — email sales@subodhtrading.com with your purchase details or column lot number.
Hypersil GOLD C4 — Ultra-pure silica C4, USP L26, ~300 Å pore size. Available in 1.9, 3, and 5 µm particle sizes across 1.0–4.6 mm ID and 50–250 mm lengths.
Hypersil GOLD C4, 1.9 µm, 100 × 2.1 mm. Mobile Phase: 110:2 ACN:20 mM formate. Temperature: 30°C. Flow Rate: 0.5 mL/min. Injection Volume: NL. Detection: UV 205 nm.
