FAQs — Hypersil GOLD C8 Column
Q: What is the Hypersil GOLD C8 column and what is it used for?
Hypersil GOLD C8 is an endcapped, ultra-pure silica reversed-phase HPLC column engineered for analytes with medium hydrophobicity — delivering similar selectivity to C18 with reduced retention for faster separations, excellent peak shape, high efficiency, and outstanding sensitivity. It is well suited for carotenoid analysis, moderately hydrophobic pharmaceutical compound assays, and any application where a C18 phase over-retains or extends run times unnecessarily.
Q: How does Hypersil GOLD C8 differ from Hypersil GOLD C18?
Hypersil GOLD C8 provides similar selectivity to Hypersil GOLD C18 but with lower hydrophobic retention due to its shorter C8 alkyl chain. This allows moderately hydrophobic compounds to elute quicker, enabling faster separations and shorter gradient windows. Both columns are built on the same ultra-pure silica endcapping platform and deliver the same exceptional peak shape and low tailing that define the Hypersil GOLD range. The C18 is classified under USP L1; the C8 is classified under USP L7.
Q: What makes Hypersil GOLD C8 different from a conventional C8 column?
Hypersil GOLD C8 uses the same ultra-pure silica endcapping strategy as the Hypersil GOLD C18 — engineered to deliver a significant reduction in peak tailing compared to conventional silica-based C8 columns. Conventional C8 phases on standard silica can still exhibit residual silanol activity; the Hypersil GOLD ultra-pure silica minimizes these interactions for cleaner peak shapes across basic, neutral, and acidic analytes.
Q: What particle sizes are available for Hypersil GOLD C8?
Hypersil GOLD C8 is available in three analytical particle sizes: 1.9 µm (UHPLC format), 3 µm (HPLC), and 5 µm (HPLC) — covering the full range from fast sub-minute UHPLC methods to standard analytical HPLC separations.
Q: What was demonstrated in the β-carotene application chromatogram?
The β-carotene application demonstrates the separation of three carotenoid analytes: Lutein, Lycopene, and β-Carotene on a Hypersil GOLD C8, 5 µm, 150 × 4.6 mm column using 100% MeOH mobile phase at 1.5 mL/min, 30°C, with UV detection at 450 nm. All conditions are sourced directly from the manufacturer's application chromatogram legend.
Q: What method conditions were used in the β-carotene chromatogram?
Column — Hypersil GOLD C8, 5 µm, 150 × 4.6 mm; Mobile Phase — MeOH; Temperature — 30°C; Flow Rate — 1.5 mL/min; Detection — UV 450 nm; Analytes — Lutein, Lycopene, β-Carotene. All values are sourced directly from the manufacturer's product literature.
Q: What USP classification does Hypersil GOLD C8 carry?
Hypersil GOLD C8 is classified under USP L7, as stated in the manufacturer's product literature. This supports its use in pharmacopoeial methods and regulated laboratory workflows specifying a USP L7 C8 stationary phase.
Q: What pH range and pore size does Hypersil GOLD C8 operate within?
Hypersil GOLD C8 is designed to operate across a pH range of approximately 2 to 11, as indicated in the manufacturer's phase property chart. The pore size is approximately 150 Å. Always refer to the column care and use instructions supplied with your column for precise operating limits.
Q: What column lengths and internal diameters are available for Hypersil GOLD C8?
The 1.9 µm UHPLC format is available in 30–150 mm lengths across 1.0, 2.1, and 3.0 mm ID. The 3 µm format covers 50–150 mm lengths across 1.0, 2.1, 3.0, and 4.6 mm ID. The 5 µm format covers 50–250 mm lengths across 1.0, 2.1, 3.0, 4.0, and 4.6 mm ID. Not all length and ID combinations are available for every particle size — see the Variants & Ordering tab for the complete matrix.
Q: Is a guard column available for Hypersil GOLD C8?
Yes. Drop-in Guard cartridges (4/pk, 10 mm) are available for 3 µm and 5 µm columns across multiple ID formats. For the 3 µm phase, a 30 mm × 4.6 mm ID Drop-in Guard (25203-034630) is also available. Uniguard Drop-in Guard Cartridge Holders are available: 851-00 (1.0 mm ID), 852-00 (2.1 mm / 3.0 mm ID), and 850-00 (4.0 mm / 4.6 mm ID).
Q: Can I transfer an existing Hypersil GOLD C18 method directly to Hypersil GOLD C8?
Hypersil GOLD C8 is designed to deliver similar selectivity to Hypersil GOLD C18 with reduced retention. Method transfer from C18 to C8 is often feasible with adjustments to gradient composition or run time to account for the earlier elution of moderately hydrophobic analytes. Contact Subodh Trading Company at sales@subodhtrading.com for method transfer support.
Q: Where can I buy Hypersil GOLD C8 columns in Maharashtra?
Hypersil GOLD C8 columns are available in Maharashtra through Subodh Trading Company, the authorized Thermo Fisher distributor for Maharashtra. Subodh Trading Company supplies genuine Thermo Fisher products with full warranty and Certificate of Analysis, serving laboratories across Pune, Nagpur, Nashik, Aurangabad, Chhatrapati Sambhaji Nagar, Solapur, Sangli, Kolhapur, Satara, Ahmednagar, Jalgaon, Nanded, Amravati, Akola, Latur, Ratnagiri, Thane, and all of Maharashtra. Contact: sales@subodhtrading.com.
Q: How do I obtain a Certificate of Analysis for my Hypersil GOLD C8 column?
A Certificate of Analysis is available for every Hypersil GOLD C8 column by part number and lot number from the Thermo Fisher CoA search portal at thermofisher.com. Subodh Trading Company can also assist customers in Maharashtra — email sales@subodhtrading.com with your purchase details or column lot number.
Hypersil GOLD C8 — Ultra-pure silica C8, USP L7, ~150 Å pore size. Available in 1.9, 3, and 5 µm particle sizes across 1.0–4.6 mm ID and 30–250 mm lengths.
Hypersil GOLD C8, 5 µm, 150 × 4.6 mm. Mobile Phase: MeOH. Temperature: 30°C. Flow Rate: 1.5 mL/min. Detection: UV 450 nm.
