FAQs — Hypersil GOLD CN Column
Q: What is the Hypersil GOLD CN column and what is it used for?
Hypersil GOLD CN is a cyano (nitrile) bonded HPLC column engineered for both normal-phase and reversed-phase separations, providing alternative selectivity with lower hydrophobicity than C18 or C8. It is designed for organic acid analysis, substituted aromatic compound separations, polar pharmaceutical impurity profiling, and any application where lower hydrophobicity, dual-mode capability, or orthogonal selectivity to C18 is required.
Q: Can Hypersil GOLD CN be used in both reversed-phase and normal-phase modes?
Yes. Hypersil GOLD CN is designed for both normal-phase and reversed-phase separations — a key advantage over standard alkyl phases which are restricted to reversed-phase operation. In reversed-phase mode it provides alternative selectivity with lower hydrophobicity; in normal-phase mode it retains polar compound selectivity without requiring fully non-polar solvent systems.
Q: How does the selectivity of Hypersil GOLD CN differ from C18?
Hypersil GOLD CN provides alternative selectivity to C18 through a combination of dipole-dipole interaction, pi-pi interaction, and reduced hydrophobic retention. The cyano group introduces polar interaction mechanisms absent in pure alkyl phases — meaning compounds that co-elute on C18 may be resolved on CN, and the elution order may differ. The low carbon load (4%) also means analytes elute faster than on C18, shortening run times.
Q: What analytes were demonstrated in the organic acids application chromatogram?
Five substituted benzoic acid analytes were separated: 4-Fluorobenzoic acid, o-Toluic Acid, p-Toluic Acid, 2,4,6-Trimethylbenzoic Acid, and 2,5-Dimethylbenzoic Acid — on Hypersil GOLD CN, 5 µm, 150 × 4.6 mm under isocratic reversed-phase conditions. All conditions are sourced directly from the manufacturer's application chromatogram legend.
Q: What method conditions were used in the organic acids chromatogram?
Column — Hypersil GOLD CN, 5 µm, 150 × 4.6 mm; Mobile Phase A — 25 mM KH₂PO₄, pH 2; Mobile Phase B — MeOH; Temperature — 25°C; Flow Rate — 1.5 mL/min; Detection — UV 230 nm; Analytes — 4-Fluorobenzoic acid, o-Toluic Acid, p-Toluic Acid, 2,4,6-Trimethylbenzoic Acid, 2,5-Dimethylbenzoic Acid. All values are sourced directly from the manufacturer's application chromatogram legend.
Q: What USP classification does Hypersil GOLD CN carry?
Hypersil GOLD CN is classified under USP L10, as stated in the manufacturer's product literature. This supports its use in pharmacopoeial methods and regulated laboratory workflows specifying a USP L10 cyano stationary phase.
Q: What are the pore size, carbon load, and pH range of Hypersil GOLD CN?
Hypersil GOLD CN has a pore size of 175 Å, a carbon load of 4%, and a pH operating range of 2 to 8, as stated in the manufacturer's product literature. The low 4% carbon load contributes to the lower hydrophobicity and faster elution characteristics of the CN phase. Always refer to the column care and use instructions supplied with your column for precise operating limits.
Q: What particle sizes are available for Hypersil GOLD CN?
Hypersil GOLD CN is available in three analytical particle sizes: 1.9 µm (UHPLC format), 3 µm (HPLC), and 5 µm (HPLC) — covering fast UHPLC methods through to standard analytical HPLC separations.
Q: What column lengths and internal diameters are available for Hypersil GOLD CN?
The 1.9 µm UHPLC format is available in 50–200 mm lengths in 2.1 mm ID only. The 3 µm format covers 50–150 mm lengths across 1.0, 2.1, 3.0, and 4.6 mm ID. The 5 µm format covers 50–250 mm lengths across 2.1, 3.0, 4.0, and 4.6 mm ID. Not all combinations are available for every particle size — see the Variants & Ordering tab for the complete matrix.
Q: Is a guard column available for Hypersil GOLD CN?
Yes. Drop-in Guard cartridges (4/pk, 10 mm) are available for 3 µm columns in 1.0, 2.1, 3.0, and 4.6 mm ID, and for 5 µm columns in 2.1, 3.0, 4.0, and 4.6 mm ID. Uniguard Drop-in Guard Cartridge Holders are available: 851-00 (1.0 mm ID), 852-00 (2.1 mm / 3.0 mm ID), and 850-00 (4.0 mm / 4.6 mm ID).
Q: Can Hypersil GOLD CN be used as an orthogonal selectivity column alongside C18?
Yes. Hypersil GOLD CN provides orthogonal selectivity to C18 through its combined dipole-dipole, pi-pi, and hydrophobic interaction mechanisms. For complex mixtures where C18 co-elutes compounds, CN often resolves the co-eluting pairs due to its different interaction profile. Its dual reversed-phase and normal-phase capability also allows direct comparison of both modes within a single method development workflow.
Q: Where can I buy Hypersil GOLD CN columns in Maharashtra?
Hypersil GOLD CN columns are available in Maharashtra through Subodh Trading Company, the authorized Thermo Fisher distributor for Maharashtra. Subodh Trading Company supplies genuine Thermo Fisher products with full warranty and Certificate of Analysis, serving laboratories across Pune, Nagpur, Nashik, Aurangabad, Chhatrapati Sambhaji Nagar, Solapur, Sangli, Kolhapur, Satara, Ahmednagar, Jalgaon, Nanded, Amravati, Akola, Latur, Ratnagiri, Thane, and all of Maharashtra. Contact: sales@subodhtrading.com.
Q: How do I obtain a Certificate of Analysis for my Hypersil GOLD CN column?
A Certificate of Analysis is available for every Hypersil GOLD CN column by part number and lot number from the Thermo Fisher CoA search portal at thermofisher.com. Subodh Trading Company can also assist customers in Maharashtra — email sales@subodhtrading.com with your purchase details or column lot number.
Hypersil GOLD CN — 1.9, 3, and 5 µm particle sizes, USP L10, 175 Å pore size, 4% carbon load, pH 2–8.
Hypersil GOLD CN, 5 µm, 150 × 4.6 mm. Mobile Phase A: 25 mM KH₂PO₄, pH 2. Mobile Phase B: MeOH. Temperature: 25°C. Flow Rate: 1.5 mL/min. Detection: UV 230 nm.
