FAQs — Hypersil GOLD PFP Column
Q: What is the Hypersil GOLD PFP column and what is it used for?
Hypersil GOLD PFP is a pentafluorophenyl reversed-phase HPLC column engineered for unique tri-modal selectivity — enhanced pi-pi interactions with aromatic molecules, extra retention for halogenated species, and selectivity for non-halogenated polar compounds. It is designed for polyphenol analysis, halogenated compound separations, aromatic pharmaceutical impurity profiling, and as an orthogonal selectivity tool where standard C18 fails to resolve complex mixtures.
Q: What does PFP stand for and what makes the phase unique?
PFP stands for pentafluorophenyl — a phenyl ring with five fluorine atoms substituted around it. The introduction of fluorine into the stationary phase causes significant changes in solute-stationary phase interaction compared to standard phenyl or alkyl phases. The electron-withdrawing fluorine atoms enhance pi-pi interactions with aromatic analytes, create affinity for halogenated species, and provide unique selectivity for polar compounds — three independent selectivity mechanisms in one stationary phase.
Q: How does Hypersil GOLD PFP differ from a standard phenyl column?
A standard phenyl phase provides pi-pi interactions with aromatic analytes through the unfluorinated phenyl ring. Hypersil GOLD PFP goes further — the five fluorine atoms around the phenyl ring increase electron deficiency, enhancing pi-pi interaction strength with aromatic analytes, adding halogen affinity for halogenated species, and modifying polar compound selectivity. The result is a significantly different and more versatile selectivity profile than a standard phenyl phase.
Q: What is the selectivity advantage of Hypersil GOLD PFP for halogenated compounds?
The fluorinated phenyl surface of Hypersil GOLD PFP has a natural affinity for halogenated analytes — chlorinated, fluorinated, and brominated compounds experience extra retention compared to a C18 or standard phenyl phase. This makes Hypersil GOLD PFP particularly useful for halogenated pesticide screening, chlorinated pharmaceutical impurity analysis, and environmental monitoring of halogenated contaminants.
Q: What analytes were demonstrated in the polyphenols application chromatogram?
Four polyphenol analytes were separated: Pyrogallol, Hydroquinone, Resorcinol, and Phenol, on Hypersil GOLD PFP, 1.9 µm, 50 × 2.1 mm, using 0.1% Acetic Acid isocratic mobile phase at 0.5 mL/min, 25°C, with UV detection at 280 nm. All conditions are sourced directly from the manufacturer's application chromatogram legend.
Q: What method conditions were used in the polyphenols chromatogram?
Column — Hypersil GOLD PFP, 1.9 µm, 50 × 2.1 mm; Mobile Phase — 0.1% Acetic Acid; Temperature — 25°C; Flow Rate — 0.5 mL/min; Injection Volume — 0.5 µL; Detection — UV 280 nm; Analytes — Pyrogallol, Hydroquinone, Resorcinol, Phenol. All values are sourced directly from the manufacturer's application chromatogram legend.
Q: What USP classification does Hypersil GOLD PFP carry?
Hypersil GOLD PFP is classified under USP L43, as stated in the manufacturer's product literature. This supports its use in pharmacopoeial methods and regulated workflows specifying a USP L43 pentafluorophenyl stationary phase.
Q: What are the pore size, carbon load, and pH range of Hypersil GOLD PFP?
Hypersil GOLD PFP has a pore size of 175 Å, a carbon load of 8%, and a pH operating range of 2 to 8, as stated in the manufacturer's product literature. Always refer to the column care and use instructions supplied with your column for precise operating limits.
Q: What particle sizes are available for Hypersil GOLD PFP?
Hypersil GOLD PFP is available in three analytical particle sizes: 1.9 µm (UHPLC format), 3 µm (HPLC), and 5 µm (HPLC) — covering fast UHPLC sub-minute methods through to standard analytical HPLC separations.
Q: What column lengths and internal diameters are available for Hypersil GOLD PFP?
The 1.9 µm UHPLC format is available in 20–200 mm lengths across 1.0, 2.1, 3.0, and 4.6 mm ID. The 3 µm format covers 30–150 mm lengths across 1.0, 2.1, 3.0, and 4.6 mm ID. The 5 µm format covers 50–250 mm lengths across 2.1, 3.0, and 4.6 mm ID. Not all length and ID combinations are available for every particle size — see the Variants & Ordering tab for the complete matrix.
Q: Is a guard column available for Hypersil GOLD PFP?
Yes. Drop-in Guard cartridges (4/pk, 10 mm) are available for 3 µm columns in 1.0, 2.1, 3.0, and 4.6 mm ID, and for 5 µm columns in 2.1 and 4.6 mm ID. Uniguard Drop-in Guard Cartridge Holders are available: 851-00 (1.0 mm ID), 852-00 (2.1 mm ID), and 850-00 (4.6 mm ID).
Q: Can Hypersil GOLD PFP be used as an orthogonal selectivity column alongside C18?
Yes. Hypersil GOLD PFP is designed to provide orthogonal selectivity to standard C18 phases through its unique tri-modal interaction mechanism. For complex mixtures where C18 fails to resolve all components, running the same sample on Hypersil GOLD PFP often separates co-eluting pairs — making it a valuable second-dimension or method development tool alongside Hypersil GOLD C18.
Q: Where can I buy Hypersil GOLD PFP columns in Maharashtra?
Hypersil GOLD PFP columns are available in Maharashtra through Subodh Trading Company, the authorized Thermo Fisher distributor for Maharashtra. Subodh Trading Company supplies genuine Thermo Fisher products with full warranty and Certificate of Analysis, serving laboratories across Pune, Nagpur, Nashik, Aurangabad, Chhatrapati Sambhaji Nagar, Solapur, Sangli, Kolhapur, Satara, Ahmednagar, Jalgaon, Nanded, Amravati, Akola, Latur, Ratnagiri, Thane, and all of Maharashtra. Contact: sales@subodhtrading.com.
Q: How do I obtain a Certificate of Analysis for my Hypersil GOLD PFP column?
A Certificate of Analysis is available for every Hypersil GOLD PFP column by part number and lot number from the Thermo Fisher CoA search portal at thermofisher.com. Subodh Trading Company can also assist customers in Maharashtra — email sales@subodhtrading.com with your purchase details or column lot number.
Hypersil GOLD PFP — 1.9, 3, and 5 µm particle sizes, USP L43, 175 Å pore size, 8% carbon load, pH 2–8.
Hypersil GOLD PFP, 1.9 µm, 50 × 2.1 mm. Mobile Phase: 0.1% Acetic Acid. Temperature: 25°C. Flow Rate: 0.5 mL/min. Injection Volume: 0.5 µL. Detection: UV 280 nm.
