FAQs — Hypersil GOLD Phenyl Column
Q: What is the Hypersil GOLD Phenyl column and what is it used for?
Hypersil GOLD Phenyl is a C4-linked phenyl reversed-phase HPLC column engineered for enhanced pi-pi interactions with aromatic molecules, moderate hydrophobicity, and outstanding peak shape and sensitivity. It is designed for antidepressant and benzodiazepine analysis, phthalate ester separations, hydroxylated pharmaceutical compound profiling, and any application where a phenyl phase selectivity profile provides better resolution of aromatic analytes than standard C18.
Q: What is the role of the C4 linker in Hypersil GOLD Phenyl?
The C4 linker connects the phenyl ring to the silica surface with greater conformational flexibility than a short direct-bonded linker. This allows the phenyl ring to align more effectively with aromatic analyte molecules — maximising the pi-pi interaction contribution to retention and selectivity. The result is enhanced aromatic selectivity compared to phenyl phases with shorter or more rigid linker chemistry.
Q: How does Hypersil GOLD Phenyl differ from Hypersil GOLD PFP?
Both phases provide enhanced pi-pi interactions with aromatics, but through different mechanisms. Hypersil GOLD PFP uses fluorine substituents on the phenyl ring to increase electron deficiency and enhance pi-pi interaction, while also providing halogen affinity and specific polar compound selectivity — giving it a tri-modal selectivity profile. Hypersil GOLD Phenyl uses the C4 linker for superior ring alignment and provides moderate hydrophobicity alongside pi-pi selectivity. For purely aromatic applications, either phase may be suitable; for halogenated compounds, Hypersil GOLD PFP is preferred.
Q: What analytes were demonstrated in the antidepressants application chromatogram?
Eight analytes were separated: Uracil, Acetaminophen, p-Hydroxybenzoic acid, o-Hydroxybenzoic acid, an impurity peak, Oxazepam, Diazepam, Di-isopropyl phthalate, and Di-n-propyl phthalate — on Hypersil GOLD Phenyl, 1.9 µm, 50 × 2.1 mm under a fast gradient UHPLC method. All conditions are sourced directly from the manufacturer's application chromatogram legend.
Q: What method conditions were used in the antidepressants chromatogram?
Column — Hypersil GOLD Phenyl, 1.9 µm, 50 × 2.1 mm; Mobile Phase A — 0.1% Formic acid; Mobile Phase B — 0.1% Formic acid in MeCN; Gradient — 10–60% B in 3.4 min, 60–90% B in 0.24 min; Temperature — 60°C; Flow Rate — 0.5 mL/min; Injection Volume — 0.7 µL; Detection — UV 225 and 254 nm. All values are sourced directly from the manufacturer's application chromatogram legend.
Q: What USP classification does Hypersil GOLD Phenyl carry?
Hypersil GOLD Phenyl is classified under USP L11, as stated in the manufacturer's product literature. This supports its use in pharmacopoeial methods and regulated laboratory workflows specifying a USP L11 phenyl stationary phase.
Q: What are the pore size, carbon load, and pH range of Hypersil GOLD Phenyl?
Hypersil GOLD Phenyl has a pore size of 175 Å, a carbon load of 8%, and a pH operating range of 2 to 8, as stated in the manufacturer's product literature. Always refer to the column care and use instructions supplied with your column for precise operating limits.
Q: What particle sizes are available for Hypersil GOLD Phenyl?
Hypersil GOLD Phenyl is available in three analytical particle sizes: 1.9 µm (UHPLC format), 3 µm (HPLC), and 5 µm (HPLC) — covering fast UHPLC methods through to standard analytical HPLC separations.
Q: What column lengths and internal diameters are available for Hypersil GOLD Phenyl?
The 1.9 µm UHPLC format is available in 50–200 mm lengths across 2.1, 3.0, and 4.6 mm ID. The 3 µm format covers 50–150 mm lengths across 1.0, 2.1, 3.0, and 4.6 mm ID. The 5 µm format covers 50–250 mm lengths across 2.1, 3.0, and 4.6 mm ID. Not all length and ID combinations are available for every particle size — see the Variants & Ordering tab for the complete matrix.
Q: Is a guard column available for Hypersil GOLD Phenyl?
Yes. Drop-in Guard cartridges (4/pk, 10 mm) are available for 3 µm columns in 2.1, 3.0, and 4.6 mm ID, and for 5 µm columns in 2.1, 3.0, and 4.6 mm ID. Uniguard Drop-in Guard Cartridge Holders are available: 851-00 (1.0 mm ID), 852-00 (2.1 mm / 3.0 mm ID), and 850-00 (4.6 mm ID).
Q: Can Hypersil GOLD Phenyl be used as an orthogonal selectivity column alongside C18?
Yes. Hypersil GOLD Phenyl provides a different selectivity mechanism from C18 — driven by pi-pi interaction and moderate hydrophobicity rather than purely hydrophobic retention. For complex aromatic mixtures where C18 co-elutes compounds, Hypersil GOLD Phenyl often resolves the co-eluting pairs. It is well suited as a second column in orthogonal method development alongside Hypersil GOLD C18.
Q: Where can I buy Hypersil GOLD Phenyl columns in Maharashtra?
Hypersil GOLD Phenyl columns are available in Maharashtra through Subodh Trading Company, the authorized Thermo Fisher distributor for Maharashtra. Subodh Trading Company supplies genuine Thermo Fisher products with full warranty and Certificate of Analysis, serving laboratories across Pune, Nagpur, Nashik, Aurangabad, Chhatrapati Sambhaji Nagar, Solapur, Sangli, Kolhapur, Satara, Ahmednagar, Jalgaon, Nanded, Amravati, Akola, Latur, Ratnagiri, Thane, and all of Maharashtra. Contact: sales@subodhtrading.com.
Q: How do I obtain a Certificate of Analysis for my Hypersil GOLD Phenyl column?
A Certificate of Analysis is available for every Hypersil GOLD Phenyl column by part number and lot number from the Thermo Fisher CoA search portal at thermofisher.com. Subodh Trading Company can also assist customers in Maharashtra — email sales@subodhtrading.com with your purchase details or column lot number.
Hypersil GOLD Phenyl — 1.9, 3, and 5 µm particle sizes, USP L11, 175 Å pore size, 8% carbon load, pH 2–8.
Hypersil GOLD Phenyl, 1.9 µm, 50 × 2.1 mm. Mobile Phase A: 0.1% Formic acid. Mobile Phase B: 0.1% Formic acid in MeCN. Gradient: 10–60% B in 3.4 min, 60–90% B in 0.24 min. Temperature: 60°C. Flow Rate: 0.5 mL/min. Injection Volume: 0.7 µL. Detection: UV 225 and 254 nm.
