FAQs — Hypersil GOLD SAX Column
Q: What is the Hypersil GOLD SAX column and what is it used for?
Hypersil GOLD SAX is a highly stable quaternary amine strong anion exchange HPLC column engineered for high stability to aqueous and low pH mobile phases — ideally suited for the analysis of smaller organic molecules including nucleotides and organic acids. It delivers outstanding peak shape and sensitivity for strong anion exchange applications that are beyond the capability of standard reversed-phase or weak anion exchange columns.
Q: What is the difference between SAX (strong anion exchange) and AX (weak anion exchange)?
The key difference is the nature of the charged ligand. Hypersil GOLD SAX uses a quaternary amine ligand — a permanently positively charged group whose retention capacity is independent of mobile phase pH. Hypersil GOLD AX uses a polymeric amine ligand whose charge state depends on pH, making it a weak anion exchanger. For strong anions requiring pH-independent retention under aqueous low pH conditions, SAX is the correct choice; for HILIC-mode operation and multiply charged species under broader pH conditions, AX may be preferred.
Q: Why is Hypersil GOLD SAX specifically recommended for aqueous and low pH mobile phases?
Many strong anion exchange applications — including nucleotide and organic acid analysis — require fully aqueous or near-aqueous mobile phases at low pH (pH 2–4). Hypersil GOLD SAX is engineered for high stability under these conditions, which can be aggressive for columns not specifically designed for aqueous low pH operation. This stability ensures reproducible retention and long column lifetime in demanding aqueous ion exchange workflows.
Q: What compound classes are ideally suited for Hypersil GOLD SAX?
Hypersil GOLD SAX is ideally suited for smaller organic molecules including nucleotides (AMP, GMP, CMP, UMP and their phosphorylated variants), nucleosides, organic acids, and small inorganic and organic anions. It is particularly effective for analytes requiring permanent positive charge on the stationary phase for consistent retention — compound classes where weak anion exchange phases provide insufficient or variable retention.
Q: What was demonstrated in the monophosphates application chromatogram?
The monophosphates application demonstrates Hypersil GOLD SAX retaining and separating nucleotide monophosphates under fully aqueous low pH conditions using 50 mM KH₂PO₄ (pH 3) mobile phase at 40°C, 1.0 mL/min, with UV detection at 254 nm on a 5 µm, 150 × 4.6 mm column. All conditions are sourced directly from the manufacturer's application chromatogram legend.
Q: What USP classification does Hypersil GOLD SAX carry?
Hypersil GOLD SAX is classified under USP L14, as stated in the manufacturer's product literature. This supports its use in pharmacopoeial methods and regulated laboratory workflows specifying a USP L14 strong anion exchange stationary phase.
Q: What are the pore size, carbon load, and pH range of Hypersil GOLD SAX?
Hypersil GOLD SAX has a pore size of 175 Å, a carbon load of approximately 2.5%, and a pH operating range of 2 to 8, as stated in the manufacturer's product literature. Always refer to the column care and use instructions supplied with your column for precise operating limits.
Q: What particle sizes are available for Hypersil GOLD SAX?
Hypersil GOLD SAX is available in three analytical particle sizes: 1.9 µm (UHPLC format), 3 µm (HPLC), and 5 µm (HPLC) — covering fast UHPLC sub-minute methods through to standard analytical HPLC separations.
Q: What column lengths and internal diameters are available for Hypersil GOLD SAX?
The 1.9 µm UHPLC format is available in 50–150 mm lengths in 2.1 mm ID only. The 3 µm format covers 50–150 mm lengths across 2.1, 3.0, and 4.6 mm ID, though the 50 mm is only listed in 2.1 mm ID. The 5 µm format covers 50–250 mm lengths across 2.1, 3.0, and 4.6 mm ID. See the Variants & Ordering tab for the complete part number matrix.
Q: Is a guard column available for Hypersil GOLD SAX?
Yes. Drop-in Guard cartridges (4/pk, 10 mm) are available for 3 µm columns in 2.1 and 4.6 mm ID, and for 5 µm columns in 2.1, 3.0, and 4.6 mm ID. Uniguard Drop-in Guard Cartridge Holders are available: 852-00 (2.1 mm / 3.0 mm ID) and 850-00 (4.6 mm ID).
Q: What are the part numbers for the 150 mm Hypersil GOLD SAX columns?
The 150 mm Hypersil GOLD SAX HPLC columns are available under: 26303-152130 (3 µm, 2.1 mm ID), 26303-153030 (3 µm, 3.0 mm ID), 26303-154630 (3 µm, 4.6 mm ID), 26305-152130 (5 µm, 2.1 mm ID), and 26305-154630 (5 µm, 4.6 mm ID). All part numbers are sourced directly from manufacturer product literature.
Q: Where can I buy Hypersil GOLD SAX columns in Maharashtra?
Hypersil GOLD SAX columns are available in Maharashtra through Subodh Trading Company, the authorized Thermo Fisher distributor for Maharashtra. Subodh Trading Company supplies genuine Thermo Fisher products with full warranty and Certificate of Analysis, serving laboratories across Pune, Nagpur, Nashik, Aurangabad, Chhatrapati Sambhaji Nagar, Solapur, Sangli, Kolhapur, Satara, Ahmednagar, Jalgaon, Nanded, Amravati, Akola, Latur, Ratnagiri, Thane, and all of Maharashtra. Contact: sales@subodhtrading.com.
Q: How do I obtain a Certificate of Analysis for my Hypersil GOLD SAX column?
A Certificate of Analysis is available for every Hypersil GOLD SAX column by part number and lot number from the Thermo Fisher CoA search portal at thermofisher.com. Subodh Trading Company can also assist customers in Maharashtra — email sales@subodhtrading.com with your purchase details or column lot number.
Hypersil GOLD SAX — Quaternary amine strong anion exchange, USP L14, 175 Å pore size, 2.5% carbon load, pH 2–8. Available in 1.9, 3, and 5 µm particle sizes.
Hypersil GOLD SAX, 5 µm, 150 × 4.6 mm. Mobile Phase: Aqueous KH₂PO₄ (50 mM, pH 3). Temperature: 40°C. Flow Rate: 1.0 mL/min. Injection Volume: 10 µL. Detection: UV 254 nm.
