FAQs — Hypersil GOLD Silica Column
Q: What is the Hypersil GOLD Silica column and what is it used for?
Hypersil GOLD Silica is an unbonded, highly pure base-deactivated silica normal-phase HPLC column — the same ultra-pure silica backbone that underpins the entire Hypersil GOLD bonded phase range. It is designed for normal-phase adsorption separations of steroids, lipids, fat-soluble vitamins, polar isomers, and other compound classes where normal-phase silica selectivity provides the required resolution. Outstanding peak shape and sensitivity distinguish it from conventional silica columns.
Q: What makes Hypersil GOLD Silica different from a standard unbonded silica column?
Hypersil GOLD Silica is built on the same highly pure base-deactivated silica that forms the backbone of all Hypersil GOLD columns. Standard silica columns typically have a higher level of residual silanol activity — particularly acidic silanols — that cause non-specific secondary interactions, peak tailing, and poor reproducibility. The Hypersil GOLD base-deactivation process is engineered to minimize these interactions, delivering outstanding peak shape and sensitivity that standard silica phases do not achieve.
Q: What separations is Hypersil GOLD Silica best suited for?
Hypersil GOLD Silica is best suited for normal-phase separations of steroids and steroid hormones, lipid class separations, fat-soluble vitamins, and any application requiring silica adsorption selectivity for polar isomers. It is the column of choice when classic normal-phase silica selectivity is required and bonded normal-phase phases (CN, amino) do not provide the required resolution or selectivity profile.
Q: What analytes were demonstrated in the steroids chromatogram?
Seven steroid analytes were separated in normal-phase mode: Progesterone, 21-Hydroxyprogesterone-21-acetate, 17-a-Hydroxyprogesterone, Cortisone, 11-a-Hydroxyprogesterone, Corticosterone, and Hydrocortisone — on Hypersil GOLD Silica, 5 µm, 150 × 4.6 mm, using 19:1 (v/v) hexane:ethanol mobile phase at 1.5 mL/min, 30°C, with UV detection at 254 nm. All conditions are sourced directly from the manufacturer's application chromatogram legend.
Q: What method conditions were used in the steroids chromatogram?
Column — Hypersil GOLD Silica, 5 µm, 150 × 4.6 mm; Mobile Phase — 19:1 (v/v) n-C6H14 : EtOH; Temperature — 30°C; Flow Rate — 1.5 mL/min; Injection Volume — 5 µL; Detection — UV 254 nm. All values are sourced directly from the manufacturer's application chromatogram legend.
Q: What USP classification does Hypersil GOLD Silica carry?
Hypersil GOLD Silica is classified under USP L3, as stated in the manufacturer's product literature. This supports its use in pharmacopoeial methods and regulated laboratory workflows specifying an unbonded silica stationary phase under USP L3.
Q: What is the pore size and pH range of Hypersil GOLD Silica?
Hypersil GOLD Silica has a pore size of 175 Å and a pH operating range of 2 to 8, as stated in the manufacturer's product literature. Always refer to the column care and use instructions for precise operating limits.
Q: How does Hypersil GOLD Silica compare to Hypersil GOLD CN or Amino in normal-phase mode?
All three can be used in normal-phase mode, but through different mechanisms. Hypersil GOLD Silica provides classic silica adsorption selectivity through direct silanol interaction — the traditional normal-phase mechanism. Hypersil GOLD CN and Amino provide polar interaction through their bonded groups, which modifies selectivity and generally improves reproducibility since bonded phases are less sensitive to mobile phase water content. Silica is preferred when classic adsorption selectivity is specifically required or when matching existing normal-phase silica methods.
Q: What particle sizes are available for Hypersil GOLD Silica?
Hypersil GOLD Silica is available in three analytical particle sizes: 1.9 µm (UHPLC format), 3 µm (HPLC), and 5 µm (HPLC) — covering fast UHPLC methods through to standard analytical HPLC separations.
Q: What column lengths and internal diameters are available for Hypersil GOLD Silica?
The 1.9 µm UHPLC format is available in 50–200 mm lengths in 2.1 mm ID only. The 3 µm format covers 30–150 mm lengths across 1.0, 2.1, 3.0, and 4.6 mm ID — note there is no 50 mm listed for 3 µm. The 5 µm format covers 50–250 mm lengths across 2.1, 3.0, 4.0, and 4.6 mm ID. See the Variants & Ordering tab for the complete part number matrix.
Q: Is a guard column available for Hypersil GOLD Silica?
Yes. Drop-in Guard cartridges (4/pk, 10 mm) are available for 3 µm columns in 1.0, 2.1, 3.0, and 4.6 mm ID, and for 5 µm columns in 2.1, 4.0, and 4.6 mm ID. Uniguard Drop-in Guard Cartridge Holders are available: 851-00 (1.0 mm ID), 852-00 (2.1 mm / 3.0 mm ID), and 850-00 (4.0 mm / 4.6 mm ID).
Q: Where can I buy Hypersil GOLD Silica columns in Maharashtra?
Hypersil GOLD Silica columns are available in Maharashtra through Subodh Trading Company, the authorized Thermo Fisher distributor for Maharashtra. Subodh Trading Company supplies genuine Thermo Fisher products with full warranty and Certificate of Analysis, serving laboratories across Pune, Nagpur, Nashik, Aurangabad, Chhatrapati Sambhaji Nagar, Solapur, Sangli, Kolhapur, Satara, Ahmednagar, Jalgaon, Nanded, Amravati, Akola, Latur, Ratnagiri, Thane, and all of Maharashtra. Contact: sales@subodhtrading.com.
Q: How do I obtain a Certificate of Analysis for my Hypersil GOLD Silica column?
A Certificate of Analysis is available for every Hypersil GOLD Silica column by part number and lot number from the Thermo Fisher CoA search portal at thermofisher.com. Subodh Trading Company can also assist customers in Maharashtra — email sales@subodhtrading.com with your purchase details or column lot number.
Hypersil GOLD Silica — Unbonded highly pure base-deactivated silica, USP L3, 175 Å pore size, 1.9, 3, and 5 µm particle sizes, pH 2–8.
Hypersil GOLD Silica, 5 µm, 150 × 4.6 mm. Mobile Phase: 19:1 (v/v) n-C6H14 : EtOH. Temperature: 30°C. Flow Rate: 1.5 mL/min. Injection Volume: 5 µL. Detection: UV 254 nm.
