FAQs — Hypersil GOLD Column
Q: What is the Hypersil GOLD column and what is it used for?
Hypersil GOLD is an endcapped, ultra-pure silica C18 reversed-phase HPLC column engineered for exceptional peak shape and resolution for both HPLC and LC-MS workflows. It is designed for pharmaceutical drug assays, basic compound analysis, β-blocker and bioanalytical LC-MS/MS applications, and any reversed-phase method where peak tailing of basic compounds has been a problem on conventional C18 columns.
Q: What makes Hypersil GOLD different from a standard C18 column?
Hypersil GOLD is built on ultra-pure silica with a comprehensive endcapping strategy specifically engineered to deliver a significant reduction in peak tailing compared to conventional silica-based C18 columns. This improvement in peak symmetry for basic analytes is achieved while retaining classic C18 selectivity — so existing C18 methods can often be directly transferred with improved peak quality.
Q: Which analytes benefit most from Hypersil GOLD?
Basic analytes — such as β-blockers, amines, pharmaceutical bases, and other compounds with protonatable nitrogen functionalities — benefit most from the Hypersil GOLD ultra-pure silica endcapping. Neutral and acidic analytes are also well retained with excellent peak shape, making Hypersil GOLD a general-purpose C18 column suitable for a broad range of compound classes.
Q: What particle sizes are available for Hypersil GOLD?
Hypersil GOLD analytical columns are available in three particle sizes: 1.9 µm (UHPLC format), 3 µm (HPLC), and 5 µm (HPLC). Preparative formats are available in 5 µm and 12 µm particle sizes, enabling scale-up from analytical to preparative purification within the same phase chemistry.
Q: What method conditions were used in the β-blockers application chromatogram?
The seven β-blockers application used the following conditions: Column — Hypersil GOLD, 1.9 µm, 20 × 2.1 mm; Mobile Phase A — H₂O + 0.1% formic acid; Mobile Phase B — MeCN + 0.1% formic acid; Gradient — 5 to 100% B in 0.6 min; Temperature — 20°C; Flow Rate — 400 µL/min; Detection — MS. All values are sourced directly from the manufacturer's application chromatogram legend.
Q: Which seven β-blockers were demonstrated in the application chromatogram?
The seven β-blockers separated were: Atenolol, Nadolol, Pindolol, Timolol, Metoprolol, Oxprenolol, and Propranolol — all resolved in under one minute on a 20 × 2.1 mm Hypersil GOLD 1.9 µm column.
Q: Is Hypersil GOLD compatible with LC-MS detection?
Yes. Hypersil GOLD is designed for direct LC-MS coupling. The highly organic mobile phase compatibility and low column bleed make it well suited for ESI-MS and MS/MS detection. The application chromatogram demonstrating seven β-blockers uses MS detection, confirming compatibility with LC-MS workflows.
Q: What USP classification does Hypersil GOLD carry?
Hypersil GOLD is classified under USP L1, as stated in the manufacturer's product literature. This supports its use in pharmacopoeial HPLC methods that specify a USP L1 reversed-phase C18 stationary phase.
Q: What pH range and pore size does Hypersil GOLD operate within?
Hypersil GOLD is designed to operate across a pH range of approximately 1 to 11, as indicated in the manufacturer's phase property chart. The pore size is approximately 150 Å. Always refer to the column care and use instructions supplied with your column for precise operating limits.
Q: What column lengths and internal diameters are available for analytical Hypersil GOLD?
Analytical Hypersil GOLD columns are available in lengths of 20–200 mm (1.9 µm) and 30–250 mm (3 µm and 5 µm), across internal diameters of 1.0, 2.1, 3.0, 4.0, and 4.6 mm — though not all combinations are available for every particle size. See the Variants & Ordering tab for the complete part number matrix by particle size.
Q: What preparative formats are available for Hypersil GOLD?
Hypersil GOLD preparative columns are available in 5 µm and 12 µm particle sizes. The 5 µm preparative range covers lengths of 50–250 mm with internal diameters of 10, 20, 21, and 30 mm. The 12 µm range covers lengths of 150–250 mm with internal diameters of 10, 21, 30, and 50 mm, plus a preparative guard cartridge. See the Variants & Ordering tab for full part number details.
Q: Is a guard column available for Hypersil GOLD analytical columns?
Yes. Drop-in Guard cartridges (4/pk, 10 mm) are available for 3 µm and 5 µm analytical columns across 1.0, 2.1, 3.0, 4.0, and 4.6 mm internal diameters. Uniguard Drop-in Guard Cartridge Holders are available: 851-00 (1.0 mm ID), 852-00 (2.1 mm ID), and 850-00 (4.0 and 4.6 mm ID). A dedicated preparative guard cartridge (25012-019023A) is available for the 12 µm preparative format.
Q: Where can I buy Hypersil GOLD columns in Maharashtra?
Hypersil GOLD columns are available in Maharashtra through Subodh Trading Company, the authorized Thermo Fisher distributor for Maharashtra. Subodh Trading Company supplies genuine Thermo Fisher products with full warranty and Certificate of Analysis, serving laboratories across Pune, Nagpur, Nashik, Aurangabad, Chhatrapati Sambhaji Nagar, Solapur, Sangli, Kolhapur, Satara, Ahmednagar, Jalgaon, Nanded, Amravati, Akola, Latur, Ratnagiri, Thane, and all of Maharashtra. Contact: sales@subodhtrading.com.
Q: How do I obtain a Certificate of Analysis for my Hypersil GOLD column?
A Certificate of Analysis is available for every Hypersil GOLD column by part number and lot number from the Thermo Fisher CoA search portal at thermofisher.com. Subodh Trading Company can also assist customers in Maharashtra with locating CoA documentation — email sales@subodhtrading.com with your purchase details or column lot number.
Hypersil GOLD — Ultra-pure silica C18, USP L1, ~150 Å pore size. Available in 1.9, 3, and 5 µm analytical particle sizes across 1.0–4.6 mm ID. Preparative formats available in 5 µm and 12 µm.
Hypersil GOLD 1.9 µm, 20 × 2.1 mm. Mobile Phase A: H₂O + 0.1% formic acid. Mobile Phase B: MeCN + 0.1% formic acid. Gradient: 5 to 100% B in 0.6 min. Temperature: 20°C. Flow Rate: 400 µL/min. Detection: MS.
