Hypersil Columns
Thermo Fisher — Hypersil Columns
Products
Hypersil GOLD
The Thermo Fisher™ Hypersil GOLD™ column is an endcapped, ultra-pure, silica-based reversed-phase HPLC column engineered to deliver exceptional peak shape and resolution across both HPLC and LC-MS workflows. Built on ultra-pure silica with a high-density C18 endcapped bonded phase, Hypersil GOLD is designed to deliver a significant reduction in peak tailing while retaining classic C18 selectivity — addressing the peak asymmetry challenges that have long been associated with conventional silica-based reversed-phase columns. The result is excellent resolution, efficiency, and sensitivity, with confidence in the accuracy and quality of analytical data across the full range of basic, neutral, and acidic analytes. Classified under USP L1 with a pore size of ~150 Å, Hypersil GOLD is available in three analytical particle sizes — 1.9, 3, and 5 µm — across column lengths from 20 to 250 mm and internal diameters from 1.0 to 4.6 mm, as well as dedicated preparative formats in 5 µm and 12 µm particle sizes with internal diameters up to 50 mm.
Hypersil GOLD C8
The Thermo Fisher™ Hypersil GOLD™ C8 column is an endcapped, ultra-pure silica-based reversed-phase HPLC column built on 1.9, 3, and 5 µm particle sizes — engineered for analytes with medium hydrophobicity, or when a less hydrophobic phase is required to obtain optimum retention. Designed as a direct companion to the Hypersil GOLD C18, the C8 phase delivers similar selectivity to C18 but with reduced retention — allowing moderately hydrophobic compounds to elute quicker, enabling faster separations without sacrificing the excellent peak shape, high efficiency, and outstanding sensitivity that define the Hypersil GOLD platform. The ultra-pure silica endcapping strategy is carried through to the C8 phase, ensuring the same significant reduction in peak tailing that distinguishes Hypersil GOLD from conventional silica-based C8 columns. Classified under USP L7 with a pore size of ~150 Å and a pH range of approximately 2 to 11, Hypersil GOLD C8 is available in column lengths of 30–250 mm across 1.0, 2.1, 3.0, 4.0, and 4.6 mm internal diameters, plus Drop-in Guard and Uniguard accessories.
Hypersil GOLD C4
The Thermo Fisher™ Hypersil GOLD™ C4 column is an endcapped, ultra-pure silica-based reversed-phase HPLC column built on 1.9, 3, and 5 µm particle sizes — engineered for very hydrophobic analytes where lower hydrophobicity than C18 or C8 is required to obtain optimum retention and peak shape. The shorter C4 alkyl chain delivers lower hydrophobicity than both C18 and C8 phases, allowing highly hydrophobic compounds — such as fatty acids, lipids, and large hydrophobic biomolecules — to elute under practical mobile phase conditions without excessive run times or peak broadening. As with the entire Hypersil GOLD platform, the ultra-pure silica endcapping strategy ensures excellent peak shape, high efficiency, and outstanding sensitivity across all analyte classes, including basic compounds. Classified under USP L26 with a pore size of ~300 Å and a pH range of approximately 2 to 11, Hypersil GOLD C4 is available in column lengths of 50–250 mm across 1.0, 2.1, 3.0, and 4.6 mm internal diameters, plus Drop-in Guard and Uniguard accessories.
Hypersil GOLD aQ
The Thermo Fisher™ Hypersil GOLD™ aQ column is a polar endcapped C18 reversed-phase HPLC column built on 1.9, 3, and 5 µm particle sizes — engineered to deliver a controlled interaction mechanism by which polar analytes can be retained and resolved using highly aqueous to fully aqueous mobile phases. Unlike standard C18 phases that suffer phase collapse in high-aqueous conditions, the Hypersil GOLD aQ polar endcapping is designed for stable operation in 100% aqueous mobile phases — making it the correct choice for highly polar analytes that require aqueous-rich gradients for practical retention. The polar endcapped C18 chemistry provides alternative selectivity to standard C18, enabling retention and resolution of water-soluble analytes such as vitamins, polar pharmaceutical compounds, and hydrophilic small molecules that are poorly retained on conventional reversed-phase columns. Classified under USP L1 with a pore size of 175 Å, a carbon load of 12%, and a pH range of 2 to 9, Hypersil GOLD aQ is available in column lengths of 20–250 mm across 1.0, 2.1, 3.0, 4.0, and 4.6 mm internal diameters, plus preparative formats in 5 µm with internal diameters up to 50 mm.
Hypersil GOLD PFP
The Thermo Fisher™ Hypersil GOLD™ PFP column is a pentafluorophenyl (PFP) reversed-phase HPLC column built on 1.9, 3, and 5 µm particle sizes — engineered around the principle that introduction of a fluorine group into the stationary phase causes significant changes in solute-stationary phase interaction, delivering a fundamentally different selectivity profile from standard alkyl phases. The fluorine atoms positioned around the phenyl ring enhance pi-pi interactions with aromatic molecules, while the electron-withdrawing nature of the fluorinated phase provides extra retention for halogenated species and unique selectivity for non-halogenated polar compounds — three orthogonal selectivity advantages that make Hypersil GOLD PFP a powerful complement or alternative to C18 in complex mixture separations. The polyphenol application demonstrates its ability to resolve structurally related aromatic compounds with excellent peak shape under simple aqueous isocratic conditions. Classified under USP L43 with a pore size of 175 Å, a carbon load of 8%, and a pH range of 2 to 8, Hypersil GOLD PFP is available in column lengths of 20–250 mm across 1.0, 2.1, 3.0, and 4.6 mm internal diameters.
Hypersil GOLD Phenyl
The Thermo Fisher™ Hypersil GOLD™ Phenyl column is a phenyl reversed-phase HPLC column built on 1.9, 3, and 5 µm particle sizes — engineered around a C4 linker that allows for superior alignment of the phenyl ring with aromatic molecules, delivering enhanced pi-pi interactions with aromatics that surpass what standard phenyl phases achieve. The C4 linker provides greater conformational flexibility than a direct-bonded phenyl, enabling the phenyl ring to orient more effectively toward aromatic analytes and maximise the pi-pi interaction contribution to retention and selectivity. Combined with moderate hydrophobicity and outstanding peak shape and sensitivity, Hypersil GOLD Phenyl is designed as a highly capable orthogonal selectivity tool for aromatic pharmaceutical compounds, antidepressants, phthalates, and structurally diverse analytes that benefit from a phenyl phase selectivity profile. Classified under USP L11 with a pore size of 175 Å, a carbon load of 8%, and a pH range of 2 to 8, available in column lengths of 50–250 mm across 1.0, 2.1, 3.0, and 4.6 mm internal diameters.
Hypersil GOLD CN
The Thermo Fisher™ Hypersil GOLD™ CN column is a cyano (nitrile) bonded reversed-phase and normal-phase HPLC column built on 1.9, 3, and 5 µm particle sizes — engineered for both normal-phase and reversed-phase separations, providing alternative selectivity with lower hydrophobicity than standard C18 or C8 phases. The cyano phase delivers a unique combination of dipole-dipole, pi-pi, and hydrophobic interaction mechanisms that provides alternative selectivity to alkyl phases — making it a powerful orthogonal selectivity tool for complex mixtures where standard reversed-phase methods reach their resolution limits. With lower hydrophobicity, compounds elute faster for quicker analysis, while the Hypersil GOLD ultra-pure silica platform ensures excellent peak shape and outstanding sensitivity throughout. Classified under USP L10 with a pore size of 175 Å, a carbon load of 4%, and a pH range of 2 to 8, available in column lengths of 50–250 mm across 1.0, 2.1, 3.0, 4.0, and 4.6 mm internal diameters.
Hypersil GOLD Amino
The Thermo Fisher™ Hypersil GOLD™ Amino column is a high-performance aminopropyl bonded HPLC column built on 1.9, 3, and 5 µm particle sizes — engineered to deliver excellent chromatographic properties across three distinct operating modes: weak anion exchange, reversed-phase, and normal phase. In weak anion exchange mode, the aminopropyl phase retains anions and organic acids through electrostatic interaction; in reversed-phase mode it is excellent for carbohydrate analysis, a compound class that is notoriously difficult to retain on standard C18 phases; and in normal-phase mode it provides alternative selectivity to silica columns without the variability associated with bare silica. Outstanding peak shape and sensitivity are delivered throughout all three modes by the Hypersil GOLD ultra-pure silica platform. Classified under USP L8 with a pore size of 175 Å, a carbon load of approximately 2%, and a pH range of 2 to 8, available in column lengths of 30–250 mm across 1.0, 2.1, 3.0, 4.0, and 4.6 mm internal diameters.
Hypersil GOLD AX
The Thermo Fisher™ Hypersil GOLD™ AX column is a weak anion exchange HPLC column built on 1.9, 3, and 5 µm particle sizes — engineered around a novel polymeric amine ligand bonded to highly pure base-deactivated silica, delivering a unique combination of weak anion exchange selectivity and HILIC-mode retention in a single stationary phase. Unlike traditional polymer-based ion exchange columns, the Hypersil GOLD AX bonded polymeric amine phase is designed for higher efficiency — producing sharper peaks and better resolution for multiple charged species and highly polar molecules that are inaccessible to standard reversed-phase methodology. Its dual capability makes it suitable for weak anion exchange retention of multiply charged anions and for HILIC-mode retention and separation of highly polar molecules, with outstanding peak shape and selectivity throughout both modes. With a pore size of approximately 100 Å and particle sizes of 1.9, 3, and 5 µm, Hypersil GOLD AX is available in column lengths of 30–250 mm across 1.0, 2.1, 3.0, and 4.6 mm internal diameters.
Hypersil GOLD SAX
The Thermo Fisher™ Hypersil GOLD™ SAX column is a highly stable quaternary amine strong anion exchange HPLC column built on 1.9, 3, and 5 µm particle sizes — engineered for high stability to aqueous and low pH mobile phases, making it the preferred choice for ion exchange applications that require robust performance under challenging aqueous conditions. The permanent positive charge of the quaternary amine ligand provides strong anion exchange retention that is independent of mobile phase pH — unlike weak anion exchange phases whose retention varies with protonation state. Ideally suited to the analysis of smaller organic molecules including nucleotides and organic acids, Hypersil GOLD SAX delivers outstanding peak shape and sensitivity through the Hypersil GOLD ultra-pure silica platform. Classified under USP L14 with a pore size of 175 Å, a carbon load of approximately 2.5%, and a pH range of 2 to 8, available in column lengths of 50–250 mm across 2.1, 3.0, and 4.6 mm internal diameters.
Hypersil GOLD Silica
The Thermo Fisher™ Hypersil GOLD™ Silica column is an unbonded, highly pure base-deactivated silica HPLC column built on 1.9, 3, and 5 µm particle sizes — the same ultra-pure silica backbone that underpins the entire Hypersil GOLD range of bonded phase columns. As an unbonded silica phase, it is engineered for normal-phase separations, providing adsorption-based retention through free silanol interactions for polar compound classes including steroids, lipids, fat-soluble vitamins, and structurally related isomers that require normal-phase selectivity for resolution. The highly pure base-deactivated silica minimizes non-specific secondary interactions — a key source of peak tailing and irreproducibility on conventional silica columns — delivering outstanding peak shape and sensitivity that distinguishes Hypersil GOLD Silica from standard unbonded silica phases. Classified under USP L3 with a pore size of 175 Å, a pH range of 2 to 8, and negligible carbon load, available in column lengths of 30–250 mm across 1.0, 2.1, 3.0, 4.0, and 4.6 mm internal diameters.
Hypersil GOLD HILIC
The Thermo Fisher™ Hypersil GOLD™ HILIC column is a hydrophilic interaction liquid chromatography HPLC column built on 1.9, 3, and 5 µm particle sizes — engineered to retain and separate polar analytes that are problematic using reversed-phase columns, providing alternative selectivity to C18 without the need for ion-pair reagents or derivatization. The Hypersil GOLD HILIC phase delivers improved sensitivity for MS detection through the use of high-organic HILIC mobile phases that enhance ESI ionization efficiency — making it a natural fit for LC-MS/MS bioanalytical and metabolomics workflows requiring polar analyte retention. Built on the same Hypersil GOLD ultra-pure silica platform that underpins the entire range, it delivers outstanding peak shape and selectivity for polar compound classes including urea, amino acids, nucleosides, and other hydrophilic analytes that elute at or near the void volume on standard reversed-phase columns. With a pore size of 175 Å, a carbon load of 6%, and a pH range of 2 to 8, available in column lengths of 30–250 mm across 1.0, 2.1, 3.0, and 4.6 mm internal diameters.
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